Physicochemical studies of indocyanine green (ICG): absorbance/concentration relationship, pH tolerance and assay precision in various solvents · Summary.
Oct 12, 2014 Following the intravenous injection of indocyanine green (ICG), the properties of a near-infrared laser light source and a charged-coupled
The ICG absorption spectrum has two peaks: the M and H-peaks. M-peak is the dominant peak that results from the absorption of ICG monomers, while H-peak is the second peak at the blue side that represents the aggregated forms of ICG [2, 31]. As the application of US disrupts the aggregates of ICG, more ICG monomers become available to bind with the hydrophobic core of BSA. Emission and absorption properties of indocyanine green (ICG) in Intralipid solution have been investigated. The study is focused on relatively low ICG concentration at a range of 0 to 20 microM.
ICG mode, and vice versa, is simply done via foot-pedal control. When used in NIR/ICG fluorescence mode, the KARL STORZ OPAL1 ® technology can be adjusted such that the video image appears either in blue or in green color. Using the NIR/ICG green mode offers intensive fluores-cence and detailed delineation against the surrounding tissue. To determine the ICG loading efficiency, the optical absorbance of the dialysate was measured with a UV–Vis spectrometer at 780 nm and compared against a calibration curve to calculate the amount of unbound ICG. Conjugated ICG in MoS 2 nanosheets was determined as unbound ICG subtracted from the total ICG used. ICG absorbs light energy, and the absorbed energy is converted to heat (photothermal effect) or transferred to molecular oxygen (type II photooxidation) generating singlet oxygen as a kind of reactive oxygen species (ROS) [ 1, 2, 26 IJA-to-ICG absorbance ratio (IJA/ICG) was taken as the ratio of absorbance at 892 nm to 792 nm (i.e.
The absorbance of free ICG in PBS solution was shown to rapidly decrease by 53% after 24 h and by 83% after 48 h, respectively. This decrease in UV/Vis absorbance may be due to the aqueous instability of ICG and subsequent physicochemical transformation as reported previously ( 13 ).
ICG molecules in solution remain in monomeric form at concentrations below approximately 400 μ g ∕ ml, and the absorbance spectrum shows a peak between 780 and 810 nm, depending on the solvent medium. 18 At higher concentrations, ICG forms dimers and oligomers, resulting in a new, lower absorbance peak between 680 and 730 nm, again dependent on the solvent medium.
To summarize, ICG absorption depends on its concentration and its solute. The Molar Extinction Coefficient Defined. If the transmission through a sample is given by T, then the absorbance A is.
CEP – Open Ended ICG – Antarctic Treaty Committee for Environmental. Protection Open nitrogen (DON), optical properties (absorbance. and fluorescence)
This decrease in UV/Vis absorbance may be due to the aqueous instability of ICG and subsequent physicochemical transformation as reported previously ( 13 ). Next, ICG adsorbed LAPMi-PTX (ICG/LAPMi-PTX) were collected with a 3 kDa centrifugal filter (Merck, NJ, USA) at 10000 rpm for 30 min and were washed with DW. The amount of adsorbed ICG was determined by measuring the absorbance of ICG from the filtrate. The [email protected]@ICG was incubated in cellular medium at 37 °C, and then the supernatant was collected after 2 and 24 h to investigate its release from the carrier. The performed studies revealed indeed that the ICG at a given condition was released from the nanocarrier, which is also a known phenomenon for other PDA-based nanostructures. 2013-07-12 · These levels of NETs or ICG were used to ensure the same absorbance value (0.6) at 808 nm in both NETs- and ICG-treated cells.
Uptake of MH-ICG by bacteria In this study, we used Staphylococcus aureus ( S aureus , ATCC 25923; Gram-positive bacteria) because S . 2021-02-02 · The absorbance of ICG decreased in the presence of polymersomes at 791 nm and reached 38% under irradiation, while the absorbance of the blank ICG was maintained at 86%, indicating the effective
Maximal absorbance was calculated from ICG time courses to evaluate myocardial blood flow. 2.11. Statistical Analysis. All data are presented as the mean ± standard deviation.
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The ICG release of different NPs (encapsulating 0.1 mg·mL-1 ICG) in PBS were characterized by measuring ICG absorbance in the release medium. The fluorescence variations between ICG and NPs were detected using a fluorescence spectrometer (FLSP920, Edinburgh Instruments, UK). We continuously measured hepatic absorbance of indocyanine green (ICG) using near‐infrared (NIR) spectroscopy after intravenous bolus injection in rabbits. Hepatic ICG concentration was obtained by subtracting out the absorbance of hemoglobin and other pigments within the liver.
Both the NETs- and ICG-treated cells were washed twice with PBS prior
Little absorption in the visible range accounts for the low autofluorescence, tissue absorbance, and scattering at NIR wavelengths (700-900 nm). This ICG azide can be used to label alkyne-tagged biomolecules (like proteins, lipids, nucleic acids, sugars) chemoselectively via the well known click-chemistry.
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High-performance liquid chromatography (HPLC) was used for measuring absorbance and fluorescence of ICG and its potential degradation compounds. Stability and degradation were evaluated under light exposure or in darkness at various temperatures. Under these conditions, degradation of ICG was evaluated over a period of 11 days.
It is used for determining cardiac output, hepatic function, liver and gastric blood flow, and for ophthalmic angiography. [2] It has a peak spectral absorption at about 800 nm. [3] ICG is an indicator and a weak acid with a pKa of 3.27. In bile stabilized with 25% methanol, the precision of the method (CV) is 5% and the accuracy is 106–127%.
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The O.D. (absorbance) at 280 nm is the maximum absorption of protein while 785 nm is the maximum absorption of ICG dyes. To obtain accurate DOS, make sure that the conjugate is free of the non-conjugated dye.
Considering the in vivo conditions, ICG stability of each ICG sample was also checked at 37 C. The absorbance of both of free and emulsion-loaded ICG gradually decreased during 12 days of 2018-03-29 · The ICG degrades quickly in aqueous solutions and loses its absor-bance peak at 800nm [36].